RM-EC O.157

Escherichia coli O.157 (non-VTEC) in milk

10 vials 1,5 ml

Availability: In stock

RM-EC.O157 for use as first line control for the analysis of Escherichia coli serotype O157:H7 (non-VTEC) according to ISO 16654 and for media control according to ISO 11133. The results can be plotted in the BiosistoChart according to NEN 6603.

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Product Specifications

Additional Information

Name Escherichia coli O.157 (non-VTEC) in milk
Product name RM-EC O.157
Accredited no
Type Single
Matrix Milk
Concentration Medium
Organism Bacteria
Lifestage Cell
Volume 10 vials 1,5 ml
UN number UN 3373
WDCM number WDCM 00014
NCCB number NCCB 100282
CCUG number n.a.


Introduction to Escherichia coli O157 in milk (RM-EC.O157)

The Reference Material Escherichia coli O157 (RM-EC.O157) in milk matrix contains WDCM00014; NCCB 100282 Escherichia coli serotype O157:H7 (non-VTEC) and can be used as first line control in microbial analysis for the enumeration of Escherichia coli O157, validation studies, media control and learning purposes. 

Escherichia coli O157 is a Gram-positive, rod-shaped, facultative anaerobic bacterium. This bacterium is found in the intestinal contents of cattle, but is best known for causing ‘colonic escherichiosis’. A foodborne illness caused by consumption of contaminated raw leaf green vegetables, undercooked meat and raw milk. 

The strain used in this reference material is not capable to produce the Shiga-like toxin (verotoxin).

RM-EC.O157 can be used for different methods, for example:

ISO 16654  Microbiology of food and animal feeding stuffs – Horizontal method for the detection of Escherichia coli O157

ISO 11133   Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media

NEN 6603   Environment and food – Internal quality control by the use of control charts with chemical and microbiological analysis

Results from the detection method (present or absent) as well as the results of counts on agar plates can be plotted in the BiosistoChart according to NEN 6603.


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